Coding

Part:BBa_K392030:Design

Designed by: Teoh Shao Thing, Rie Takino, Kosuke Torigata   Group: iGEM10_Osaka   (2010-10-25)


yeast secretion tag + C. fermi endoglucanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
    Illegal NotI site found at 1297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1210
    Illegal BamHI site found at 346
    Illegal XhoI site found at 708
    Illegal XhoI site found at 957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 488
    Illegal NgoMIV site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 392


Design Notes

Assembled from Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.


Source

Silver assembly of K416003 with K392006

References